Inhibition of DNA and RNA metabolism by daunorubicin and adriamycin in L1210 mouse leukemia.
نویسندگان
چکیده
Effects of daunorubicin and its new analog, adriamycin, on nucleic acid metabolism were studied in vitro in L1210 mouse leukemia cells with labeled nucleoside precursors and were compared with the established effects of actinomycin D. L1210 ascites tumor cells incubated with daunorubicin under physiological conditions of pH, temperature, and tonicity showed significantly greater inhibition of tritiated thymidine incorporation into DNA and ' 4C-labeled uridine incorporation
منابع مشابه
Inhibition of DNA and RNA Metabolism by Daunorubicin and Adriamycin in L1210 Mouse Leukemia1
Effects of daunorubicin and its new analog, adriamycin, on nucleic acid metabolism were studied in vitro in L1210 mouse leukemia cells with labeled nucleoside precursors and were compared with the established effects of actinomycin D. L1210 ascites tumor cells incubated with daunorubicin under physiological conditions of pH, temperature, and tonicity showed significantly greater inhibition of t...
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Adriamycin (AM) and daunomycin (DM) were compared as inhibitors of nucleic acid and protein biosynthesis in LI 210 leukemic cells. In vivo, both antibiotics markedly inhibited, without significant difference, the incorporation of the labeled precursors into DNA and RNA. In vitro, at a concentration of 5 or lOjUg/ml, DM was significantly more effective than AM in inhibiting DNA and RNA synthesis...
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The free radical scavengers, dimethyl sulfoxide (MezSO) and thiourea, were used to assess the role of free radicals in the production of intercalator-induced DNA breaks and cytotoxicity in mouse leukemia L1210 cells. Both agents decreased X-ray break production, and this decrease was comparable in magni tude to the degree of inhibition of X-ray-induced cell killing. By contrast, Me2SO increased...
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The free radical scavengers, dimethyl sulfoxide (Me2SO) and thiourea, were used to assess the role of free radicals in the production of intercalator-induced DNA breaks and cytotoxicity in mouse leukemia L1210 cells. Both agents decreased X-ray break production, and this decrease was comparable in magnitude to the degree of inhibition of X-ray-induced cell killing. By contrast, Me2SO increased ...
متن کاملRelationship between effects on nucleic acid synthesis in cell cultures and cytotoxicity of 4-demethoxy derivatives of daunorubicin and adriamycin.
Four new derivatives of daunorubicin and two new derivatives of Adriamycin characterized by the absence of the methoxyl groups at the C-4 position have been studied in cell cultures in vitro to establish structure-activity relationships. 4-Demethoxydaunorubicin was 27 to 100 times more active than was daunorubicin when inhibiting the cloning efficiency of exponential-phase HeLa cells and, like ...
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ورودعنوان ژورنال:
- Cancer research
دوره 32 6 شماره
صفحات -
تاریخ انتشار 1972